The SerialEM Script Repository


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Name TuneScope
Category Calibrations
Language SerialEM Scripting
Description Script to tune Polara, Talos Arctica and/or Titan Krios
Author Wim Hagen

Version History

Version 0.1


Upload date April 08, 2019
Download count 388
SerialEM version ≥ 3.7



Setup low dose.
Switch low dose off.
If needed drop the spot size one or two steps, DO NOT CHANGE BEAM SIZE!!!
Burn a carbon area clean to get nice Thon-rings.
Adjust desired defocus.
Start script and sit back.

The script:
Focus is set to zero
Loop focus measurement and set stage height to get the desired defocus
Run Zemlin tableau, iterate when out of spec.
Dialog asking to insert and center objective aperture
Autostigmate with objective aperture


This code can be shared and adapted under the Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0) License (