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Quick Tomography

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Details

Name Quick Tomography
Permalink https://serialemscripts.nexperion.net/script/42
Category Tomography
Description This script acquires a tomogram without additional tracking steps. The speedup compared to setting up a traditional tomo will typically be 30-50%. Currently works with FEI compustages only. A fast CMOS-based camera with drift/frame correction is recommended.
Author Martin Schorb
Literature reference https://doi.org/10.1038/s41592-019-0396-9

Version History

Version 2018.10

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Upload date October 10, 2018
Download count 139
SerialEM version ≥ 3.7.0-beta

            
	    	
	    		
	    

Documentation

Quick Tomography



Acquires a tomogram without additional tracking steps. We acquire tilt series from + to -60 deg with 1 deg increment in about 7 minutes using a TF30 and a OneView camera.

* The script will name the files according to the Navigator Label.

* It allows to automatically append -a or-b depending on the axis for dual tomo.

* A second tomo with lower mag and different tilt increment or defocus can be acquired right after the first in opposite tilt direction.

License

This code can be shared and adapted under the Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0) License (https://creativecommons.org/licenses/by-nc-sa/4.0/)