The SerialEM Script Repository

Focus Pair

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Details

Name Focus Pair
Permalink https://serialemscripts.nexperion.net/script/36
Category High-Level Functions
Language SerialEM Scripting
Description Acquires and saves a focus pair
Author Juha Huiskonen
Website link http://www.opic.ox.ac.uk/wiki/index.php/Macros_for_SerialEM

Version History

Version 1.0

Download

Upload date March 01, 2018
Download count 556
SerialEM version ≥ (untested) 

            
	    	
	    		
	    

	    
Documentation

                  
Preview image is used for pre-exposure (which we believe is advantageous to reduce beam induced drift due to charging effects) and for final centering of the beam. The first image is taken and defocus defined by "defocus target" and the second image is taken 3 um further from focus. If you don't need focus pairs, simply remove or uncomment the relevant lines.
        
	
License

        
This code can be shared and adapted under the Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0) License (https://creativecommons.org/licenses/by-nc-sa/4.0/)