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Check grid

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Name Check grid
Permalink https://serialemscripts.nexperion.net/script/28
Category Other Data Acquisition
Description Quick survey of TEM grid.
Author Jason de la Cruz

Version History

Version 1.0

Download

Upload date June 30, 2017
Download count 187
SerialEM version ≥ 3.7.0-beta

            
	    	
	    		
	    

Documentation

Modify settings in section below. Set "setIntensityCmd" to either "SetIlluminatedArea" for FEI Titan series or "SetPercentC2" for all other microscopes. Decide on condenser beam illumination (aperture, spot size); enter desired spot size in settings section. Then set "lowMag" to lowest mag with unobstructed view of TEM grid (i.e., the mag you use for whole-grid atlas), adjust intensity for optimum signal, center beam shift, then record intensity value in "lowMagIntensity".

When ready to start, open file to save autodoc metadata (with .mdoc or .idoc) then run the script. Close the file when done.

Please check that beam is centered in low mag before continuing.
Ensure all checkboxes in "Script > Controls..." dialog are unchecked to avoid "low counts" errors.

License

Copyright (c) 2017, Howard Hughes Medical Institute. License ISC: Internet Software Consortium license, http://opensource.org/licenses/ISC. This is free software: you are free to change and redistribute it. There is NO WARRANTY, to the extent permitted by law.