Continuous image acquisitionEdit Delete
|Name||Continuous image acquisition|
|Category||Other Data Acquisition|
|Description||This script coordinates TEM and imaging detector to collect and save a sequential set of images to disk by operating the detector in continuous imaging mode. Metadata pertinent to MicroED (https://cryoem.janelia.org/pages/MicroED) post-processing is saved in the autodoc file.|
|Author||Jason de la Cruz|
|Upload date||June 08, 2017|
|SerialEM version ≥||3.7|
Open file to save autodoc metadata (with .mdoc or .idoc). Modify recordMode, frameExposureTime, and numberFrames settings in script. Start goniometer rotation (outside of SerialEM) at a few degrees past intended start angle, then start the script to initiate continuous recording. When done, close file.
recordMode options: V (View mode), F (Focus mode), R (Record mode)
frameExposureTime is in seconds
numberFrames is the total number of frames comprising the movie
Copyright (c) 2017, Howard Hughes Medical Institute. Copying and distribution of this file, with or without modification, are permitted in any medium without royalty provided the copyright notice and this notice are preserved. This file is offered as-is, without any warranty.