The SerialEM Script Repository

Continuous image acquisition

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Name Continuous image acquisition
Category Other Data Acquisition
Language SerialEM Scripting
Description This script coordinates TEM and imaging detector to collect and save a sequential set of images to disk by operating the detector in continuous imaging mode. Metadata pertinent to MicroED ( post-processing is saved in the autodoc file.
Author Jason de la Cruz

Version History

Version 1.0


Upload date June 08, 2017
Download count 650
SerialEM version ≥ 3.7



Open file to save autodoc metadata (with .mdoc or .idoc). Modify recordMode, frameExposureTime, and numberFrames settings in script. Start goniometer rotation (outside of SerialEM) at a few degrees past intended start angle, then start the script to initiate continuous recording. When done, close file.

recordMode options: V (View mode), F (Focus mode), R (Record mode)
frameExposureTime is in seconds
numberFrames is the total number of frames comprising the movie


Copyright (c) 2017, Howard Hughes Medical Institute. Copying and distribution of this file, with or without modification, are permitted in any medium without royalty provided the copyright notice and this notice are preserved. This file is offered as-is, without any warranty.