The SerialEM Script Repository

Continuous image acquisition

Edit Delete

Details

Name Continuous image acquisition
Permalink https://serialemscripts.nexperion.net/script/26
Category Other Data Acquisition
Description This script coordinates TEM and imaging detector to collect and save a sequential set of images to disk by operating the detector in continuous imaging mode. Metadata pertinent to MicroED (https://cryoem.janelia.org/pages/MicroED) post-processing is saved in the autodoc file.
Author Jason de la Cruz

Version History

Version 1.0

Download

Upload date June 08, 2017
Download count 299
SerialEM version ≥ 3.7

            
	    	
	    		
	    

Documentation

Open file to save autodoc metadata (with .mdoc or .idoc). Modify recordMode, frameExposureTime, and numberFrames settings in script. Start goniometer rotation (outside of SerialEM) at a few degrees past intended start angle, then start the script to initiate continuous recording. When done, close file.

recordMode options: V (View mode), F (Focus mode), R (Record mode)
frameExposureTime is in seconds
numberFrames is the total number of frames comprising the movie

License

Copyright (c) 2017, Howard Hughes Medical Institute. Copying and distribution of this file, with or without modification, are permitted in any medium without royalty provided the copyright notice and this notice are preserved. This file is offered as-is, without any warranty.